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Department of Pathology
University of Pittsburgh
School of Medicine
S-417 BST
200 Lothrop Street
Pittsburgh, PA 15261
(412) 648-9550


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 Department of Pathology
 Division of Experiemental Pathology



PRINCIPAL INVESTIGATOR:

Shirish Paranjpe, PhD
- Research Assistant Professor of Pathology

Office:

S453 BST 200 Lothrop Street
Pittsburgh, PA 15261
Email: shirish@pitt.edu Tel: 412-383-8787
Fax: 412-648-1916

  

RESEARCH:

Dr. Paranjpe`s research interest lie in understanding the role of microRNA`s in control and regulation of liver growth, regeneration and differentiation. His studies focus on identification of microRNA`s that are differentially regulated in the partial hepatectomy model of liver regeneration in rats. Of particular interest is the liver-enriched microRNA rno-mir122 and the let-7 family of microRNA`s. Preliminary analysis of microRNA profile in control and regenerating liver indicated that 28 miRNAs were significantly regulated in the PHx group as compared to resting liver and sham control. We will be focusing on the liver-enriched microRNA, rno-mir122a and the let-7 family of microRNA`s to gain a better understanding of their role in liver regeneration.

Another area of interest is the role of Decorin in Liver Biology. Decorin, is a small leucine - rich proteoglycan (SLRP) is involved in regulation of cellular proliferation, matrix assembly, cell adhesion and cell migration. Decorin has been shown to suppress proliferation of cancer cells in vitro. Decorin interacts with a number of receptor tyrosine kinases like Met, the receptor for Hepatocyte Growth Factor (HGF) and Epidermal Growth Factor Receptor (EGFR) a member of the ErbB family of receptors. Of the various agents implicated in liver regeneration, HGF and ligands of the EGF receptor are the only ones that stimulate DNA synthesis in hepatocyte cultures maintained in chemically defined media. They are also the only ones that stimulate DNA synthesis in the liver of normal mice and rats. HGF and EGF signaling pathways are activated within 60 minutes following a PHx. In view of the role of Decorin as a negative regulator of Met and EGFR, is likely that Decorin has a role in hepatocyte proliferation, both in vitro and liver regeneration in vivo. Preliminary data indicated that Decorin expression, following a PHx, is significantly suppressed during early phases of liver regeneration and Decorin has a modulating role in hepatocyte proliferation

PUBLICATIONS:

  1. Sub compartmentalization of HIV-1 quasispecies between seminal cells and seminal plasma indicates their origin in distinct genital tissues. (2002). Paranjpe, S, Craigo, J., Patterson, B., Ding, M, Barroso, P., Harrison, L., Montelaro, R. & Gupta, P. AIDS Res.Hum.Retro.18:1271-1280.

  2. Persistent HIV type 1 infection in semen and blood compartments in patients after long-term potent antiretroviral therapy. Craigo, J.K., Patterson, B., Paranjpe, S., Kulka, K., Ding, M., Mellors, J., Montelaro, R.C., & Gupta, P. (2004). AIDS Res.Hum.Retro. 20:1196-1209.

  3. High throughput screening of methylation status of genes in prostate cancer using oligonucleotide methylation array. (2005). Yan Ping Yu, Paranjpe, S, Nelson, J., Finkelstein S., Ren B., Kokkinakis, D., Michalopoulos, GK. & Luo, J.H. Carcinogenesis. 26:471-479.

  4. Cell cycle effects resulting from inhibition of HGF and its receptor c-met in regenerating rat livers by RNA interference (2007). Paranjpe, S, Bowen, W.C., Bell, A.W., Kari Nejak-Bowen, Luo, J-H & Michalopoulos, G.K. Hepatology, Hepatology. 45 (6): 1471-1477.

  5. Liu B, Paranjpe S, Bowen WC, Bell AW, Luo JH, Yu YP, Mars WM, Michalopoulos GK. (2009). Investigation of the Role of Glypican 3 in Liver Regeneration and Hepatocyte Proliferation. Am J Pathol, 175:717-724.

  6. Liu B, Bell, AW, Paranjpe, S, Bowen WC, Khillan JS, Luo J-H, Mars WM, Michalopoulos GK. (2010). Suppression of Liver Regeneration & Hepatocyte Proliferation in Glypican 3 Hepatocyte -Targeted Transgenic Mice. In Press, Hepatology. PHS 398/2590 (Rev. 09/04) Page 3 Biographical Sketch Format Page

  7. Paranjpe S, Bowen WC, Tseng GC, Luo, J-H, Orr, A & Michalopoulos, GK. (2010). RNA Interference against hepatic Epidermal Growth Factor Receptor has Suppressive Effects on Liver Regeneration in Rats. Am J Pathol, 176:2669-2681.

Copyright 1995-2009, Department of Pathology
University of Pittsburgh School of Medicine